papB [2019-06-28 11:30:04]

Xaa-Pro amino-peptidase

locus: BSU13860

pI: 5.17

mw: 40.18 kDa

protein length: 363 aa

gene length: 1092 bp

function: degradation of proline-containing peptides

product: Xaa-Pro amino-peptidase

essential: no

ec: 3.4.11.9

synonyms: ykvY

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.3|Utilization of nitrogen sources other than amino acids] → [category|SW 2.3.3.4|Utilization of peptides]
• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.6|Coping with hyper-osmotic stress]

Gene

Coordinates: 1,453,691 → 1,454,782

The protein

Protein family

• peptidase M24B family (according to Swiss-Prot)

Paralogous protein(s)

• [protein|33886A1EAC13B684E92BF94201C46617AD2844B3|PapA]

Structure

• [PDB|3Q6D] (''B. anthracis'' Xaa-Pro dipeptidase, 35% identity, 55% similarity)

Expression and Regulation

Operons

• genes: [gene|C95EEAC79B9E823BBBCF6B1960DABCCBB2AC03A4|papB]
  description: [Pubmed|22383849]

• genes: [gene|C95EEAC79B9E823BBBCF6B1960DABCCBB2AC03A4|papB]-[gene|6FDE01EEFCDB2F949A902DFD76A341410B867E88|ykvZ]-[gene|BC0DF593AE969F1DBC5E9E2BACF1C00BE4FEE449|glcT]
  description: [Pubmed|22383849]

Biological materials

Mutant

• MGNA-B330 (ykvY::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1329 NBRP B. subtilis, Japan]
• BKE13860 (Δ[gene|C95EEAC79B9E823BBBCF6B1960DABCCBB2AC03A4|papB]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE13860 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACTTCTTGTTCCTCCCTGC, downstream forward: _UP4_TAATAGAAATAAAAAAGGAC
• BKK13860 (Δ[gene|C95EEAC79B9E823BBBCF6B1960DABCCBB2AC03A4|papB]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK13860 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACTTCTTGTTCCTCCCTGC, downstream forward: _UP4_TAATAGAAATAAAAAAGGAC

References

• 23144141